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Registro Completo |
Biblioteca(s): |
Embrapa Cerrados; Embrapa Soja. |
Data corrente: |
11/04/2002 |
Data da última atualização: |
11/04/2002 |
Autoria: |
MOSTASSO, L.; MOSTASSO, F. L.; DIAS, B. G.; VARGAS, M. A. T.; HUNGRIA, M. |
Título: |
Selection of bean (Phaseolus vulgaris L.) rhizobial strains for the Brazilian Cerrados. |
Ano de publicação: |
2002 |
Fonte/Imprenta: |
Field Crops Research, Amsterdam, v. 73, p. 121-132, 2002. |
Idioma: |
Inglês Português |
Conteúdo: |
The common bean croop (Phaseolus vulgaris L.) occupies 5.5 million hectares in Brazil and provides about 30% of the populations protein needs. Yield remains low mainly due to limited availability of N and P, and to the often acid soil conditions. Surprisingly, there have been only very limited studies in Brazil in which the isolation and evaluation of efficient N fixing bean strains has been attempted. This paper reports the selection of bean rhizobia for the Cerrados, the savannas that represent one-fourth of Brazilian land. Two hundred strains were selected from large pink colored nodules collected in the Cerrados region and biological nitrogen fixation was evaluted under optimal (27/21º C, day/nigth) and high (37/21º C) temperature conditions. Thirty-six strains were selected and their nitrogen-fixing capacity and competitiveness further evaluated with black-seeded Negro Argel, and colored Carioca cultivars. One fifth of the strains showed low genetic stability of nodulation gene, but some strains were as or more competitive than the strains currently recommended for use in commercial bean inoculants in Brazil The superior performance of five strains was confirmed under field conditions and reinoculation in the second year increased bean yield. The DNA fingerprintings obtained by the ERIC-REP-PCR analyses indicated a high level of genetic diversity, and among the 36 strains, six different patterns of RFLP-PCR of the 16S rRNA gene region were detected. The 16S rRNA sequences of the most efficient and competitive strains were genetically similar to Rhizobium tropici, suggesting that further studies on inoculant strains in the hot and acid soils of the Brasilian Cerrados and Africa should emphasize this species. MenosThe common bean croop (Phaseolus vulgaris L.) occupies 5.5 million hectares in Brazil and provides about 30% of the populations protein needs. Yield remains low mainly due to limited availability of N and P, and to the often acid soil conditions. Surprisingly, there have been only very limited studies in Brazil in which the isolation and evaluation of efficient N fixing bean strains has been attempted. This paper reports the selection of bean rhizobia for the Cerrados, the savannas that represent one-fourth of Brazilian land. Two hundred strains were selected from large pink colored nodules collected in the Cerrados region and biological nitrogen fixation was evaluted under optimal (27/21º C, day/nigth) and high (37/21º C) temperature conditions. Thirty-six strains were selected and their nitrogen-fixing capacity and competitiveness further evaluated with black-seeded Negro Argel, and colored Carioca cultivars. One fifth of the strains showed low genetic stability of nodulation gene, but some strains were as or more competitive than the strains currently recommended for use in commercial bean inoculants in Brazil The superior performance of five strains was confirmed under field conditions and reinoculation in the second year increased bean yield. The DNA fingerprintings obtained by the ERIC-REP-PCR analyses indicated a high level of genetic diversity, and among the 36 strains, six different patterns of RFLP-PCR of the 16S rRNA gene region were detected. The 16S rRNA sequen... Mostrar Tudo |
Palavras-Chave: |
Bean. |
Thesagro: |
Cerrado; Feijão; Fixação de Nitrogênio; Phaseolus Vulgaris; Rhizobium. |
Thesaurus Nal: |
nitrogen fixation. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02437naa a2200253 a 4500 001 1564655 005 2002-04-11 008 2002 bl --- 0-- u #d 100 1 $aMOSTASSO, L. 245 $aSelection of bean (Phaseolus vulgaris L.) rhizobial strains for the Brazilian Cerrados. 260 $c2002 520 $aThe common bean croop (Phaseolus vulgaris L.) occupies 5.5 million hectares in Brazil and provides about 30% of the populations protein needs. Yield remains low mainly due to limited availability of N and P, and to the often acid soil conditions. Surprisingly, there have been only very limited studies in Brazil in which the isolation and evaluation of efficient N fixing bean strains has been attempted. This paper reports the selection of bean rhizobia for the Cerrados, the savannas that represent one-fourth of Brazilian land. Two hundred strains were selected from large pink colored nodules collected in the Cerrados region and biological nitrogen fixation was evaluted under optimal (27/21º C, day/nigth) and high (37/21º C) temperature conditions. Thirty-six strains were selected and their nitrogen-fixing capacity and competitiveness further evaluated with black-seeded Negro Argel, and colored Carioca cultivars. One fifth of the strains showed low genetic stability of nodulation gene, but some strains were as or more competitive than the strains currently recommended for use in commercial bean inoculants in Brazil The superior performance of five strains was confirmed under field conditions and reinoculation in the second year increased bean yield. The DNA fingerprintings obtained by the ERIC-REP-PCR analyses indicated a high level of genetic diversity, and among the 36 strains, six different patterns of RFLP-PCR of the 16S rRNA gene region were detected. The 16S rRNA sequences of the most efficient and competitive strains were genetically similar to Rhizobium tropici, suggesting that further studies on inoculant strains in the hot and acid soils of the Brasilian Cerrados and Africa should emphasize this species. 650 $anitrogen fixation 650 $aCerrado 650 $aFeijão 650 $aFixação de Nitrogênio 650 $aPhaseolus Vulgaris 650 $aRhizobium 653 $aBean 700 1 $aMOSTASSO, F. L. 700 1 $aDIAS, B. G. 700 1 $aVARGAS, M. A. T. 700 1 $aHUNGRIA, M. 773 $tField Crops Research, Amsterdam$gv. 73, p. 121-132, 2002.
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5. | | SILVA, M. A. da; MILLAR, A. A. Evapotranspiracao do feijao-de-corda. In: EMBRAPA. Centro de Pesquisa Agropecuária do Trópico Semi-Árido. Pesquisa em irrigação no tropico Semi-Árido: solo, agua, planta. Petrolina, 1981. p. 15-24 (EMBRAPA-CPATSA. Boletim de Pesquisa, 4).Tipo: Capítulo em Livro Técnico-Científico |
Biblioteca(s): Embrapa Semiárido. |
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8. | | SILVA, M. A. da; PANIZZI, A. R.; LUCINI, T.; POSSEBOM, T. Biologia de ninfas e de adultos e preferência de adultos de Chinavia erythrocnemis (Heteroptera: Pentatomidae) em plantas cultivadas. In: MOSTRA DE INICIAÇÃO CIENTÍFICA, 14.; MOSTRA DE PÓS-GRADUAÇÃO DA EMBRAPA TRIGO, 11., 2019, Passo Fundo. Resumos... Brasília, DF: Embrapa, 2019. Resumos graduação PIBIC/CNPq, p. 17.Tipo: Autoria/Organização/Edição de Livros |
Biblioteca(s): Embrapa Trigo. |
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9. | | POSSEBOM, T.; PANIZZI, A. R.; LUCINI, T.; SILVA, M. A. da. Biologia de ninfas e de adultos e preferência de percevejos pentatomídeos em plantas cultivadas. In: MOSTRA DE INICIAÇÃO CIENTÍFICA, 14.; MOSTRA DE PÓS-GRADUAÇÃO DA EMBRAPA TRIGO, 11., 2019, Passo Fundo. Resumos... Brasília, DF: Embrapa, 2019. Resumos graduação, p. 31.Tipo: Autoria/Organização/Edição de Livros |
Biblioteca(s): Embrapa Trigo. |
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14. | | SILVA, M. A. da; CHAVES, L. H. G.; SILVA, D. J.; FARIA, A. F. Produtividade e qualidade do melão em função de nitrogênio, micronutrientes e matéria orgânica. Agropecuária Técnica, Areia, v. 24, n. 2, p. 131-138, 2003.Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - B |
Biblioteca(s): Embrapa Semiárido. |
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